Simultaneous determination of bile acids in rat bile and serum by high-performance liquid chromatography
H Sakakura, M Suzuki, N Kimura, H Takeda… - … of Chromatography B …, 1993 - Elsevier
A method for the simultaneous determination of bile acids in rat bile and serum by high-
performance liquid chromatography with a post-column enzymic reaction and fluorescence
detection has been developed. Without prior fractionation and alkaline hydrolysis, 26
unconjugated, glycine-and taurine-conjugated bile acids were determined. They were
separated on a reversed-phase column using a linear gradient solvent system of 200 mM
dibasic ammonium phosphate buffer (pH 7.9)—acetonitrile—methanol (73: 19: 8, v/v/v) and …
performance liquid chromatography with a post-column enzymic reaction and fluorescence
detection has been developed. Without prior fractionation and alkaline hydrolysis, 26
unconjugated, glycine-and taurine-conjugated bile acids were determined. They were
separated on a reversed-phase column using a linear gradient solvent system of 200 mM
dibasic ammonium phosphate buffer (pH 7.9)—acetonitrile—methanol (73: 19: 8, v/v/v) and …
