[HTML][HTML] The kinase activation loop is the key to mixed lineage kinase-3 activation via both autophosphorylation and hematopoetic progenitor kinase 1 phosphorylation
IWL Leung, N Lassam - Journal of Biological Chemistry, 2001 - ASBMB
We have demonstrated previously that Cdc42 induced MLK-3 homodimerization leads to
both autophosphorylation and activation of MLK-3 and postulated that autophosphorylation
is an intermediate step of MLK-3 activation following its dimerization. In this report we sought
to refine further the mechanism of MLK-3 activation and study the role of the putative kinase
activation loop in MLK-3 activation. First we mutated the three potential phosphorylation
sites in MLK-3 putative activation loop to alanine in an effort to abrogate MLK-3 …
both autophosphorylation and activation of MLK-3 and postulated that autophosphorylation
is an intermediate step of MLK-3 activation following its dimerization. In this report we sought
to refine further the mechanism of MLK-3 activation and study the role of the putative kinase
activation loop in MLK-3 activation. First we mutated the three potential phosphorylation
sites in MLK-3 putative activation loop to alanine in an effort to abrogate MLK-3 …