Frequency of the Bcl-2/IgH Rearrangement in Normal Individuals: Implications for the Monitoring of Disease in Patients With Follicular Lymphoma
KE Summers, LK Goff, AG Wilson, RK Gupta… - Journal of Clinical …, 2001 - ascopubs.org
KE Summers, LK Goff, AG Wilson, RK Gupta, TA Lister, J Fitzgibbon
Journal of Clinical Oncology, 2001•ascopubs.orgPURPOSE: To determine the incidence and frequency of the Bcl-2/IgH rearrangement in the
peripheral blood of normal individuals to define the potential complication this may pose for
the molecular monitoring of disease in patients with follicular lymphoma (FL). MATERIALS
AND METHODS: The incidence and frequency of the major breakpoint cluster region
rearrangement in DNA extracted from peripheral blood or lymphoblastoid cell lines from 481
normal individuals was determined using a TaqMan real-time polymerase chain reaction …
peripheral blood of normal individuals to define the potential complication this may pose for
the molecular monitoring of disease in patients with follicular lymphoma (FL). MATERIALS
AND METHODS: The incidence and frequency of the major breakpoint cluster region
rearrangement in DNA extracted from peripheral blood or lymphoblastoid cell lines from 481
normal individuals was determined using a TaqMan real-time polymerase chain reaction …
PURPOSE: To determine the incidence and frequency of the Bcl-2/IgH rearrangement in the peripheral blood of normal individuals to define the potential complication this may pose for the molecular monitoring of disease in patients with follicular lymphoma (FL).
MATERIALS AND METHODS: The incidence and frequency of the major breakpoint cluster region rearrangement in DNA extracted from peripheral blood or lymphoblastoid cell lines from 481 normal individuals was determined using a TaqMan real-time polymerase chain reaction assay (PE Applied Biosystems, Foster City, CA).
RESULTS: Twenty three percent of samples were positive for the Bcl-2/IgH rearrangement, with approximately 3% of these at levels of more than 1 in 104 cells.
CONCLUSION: The presence of circulating Bcl-2/IgH+ cells, other than those derived from the malignant clone, could confound the detection and quantitation of minimal residual disease in patients with FL, particularly at low levels of tumor burden.
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