Direct detection of ototoxicant-induced reactive oxygen species generation in cochlear explants
WJ Clerici, K Hensley, DL DiMartino, DA Butterfield - Hearing research, 1996 - Elsevier
The proposal that free-radical generation contributes to the ototoxicities of several chemical
agents was studied utilizing electron paramagnetic resonance (EPR) spectrometry to detect
directly ototoxicant-induced reactive oxygen species formation in cochlear tissue. Guinea
pig cochlear explants in chelexed artificial perilymph (AP; 200 μl) were exposed to an
ototoxicant or AP for 10 min. Ototoxic agents included gentamicin sulfate (4.0 mM),
kanamycin monosulfate (4.0 mM), ethacrynic acid (0.5 mM), furosemide (0.3 mM), cisplatin …
agents was studied utilizing electron paramagnetic resonance (EPR) spectrometry to detect
directly ototoxicant-induced reactive oxygen species formation in cochlear tissue. Guinea
pig cochlear explants in chelexed artificial perilymph (AP; 200 μl) were exposed to an
ototoxicant or AP for 10 min. Ototoxic agents included gentamicin sulfate (4.0 mM),
kanamycin monosulfate (4.0 mM), ethacrynic acid (0.5 mM), furosemide (0.3 mM), cisplatin …